The World Trade Center's job was to promote world trade and international business relationships. The Twin Towers are the two main structures of the World Trade Center.
Several years ago a bomb exploed in the parking garage of the World Trade Center.
When Spider-Man was transformed into a giant spider monster he used the parking garage of the World Trade Center as his lair.
As Punisher hunted Spider-Man, he was attacked by Spider-Man and was brought to the World Trade Center. Kraven later found some of Spider-Man's webbing and picked up the scent of gunpowder (from the bomb explosion) on the web and tracked the creature to the parking garage of the World Trade Center.
As Kraven arrived at the World Trade Center parking garage he freed Punisher and they both fought Man-Spider. Mariah Crawford shortly showed up in the World Trade Center parking garage and cured Spider-Man. Kraven then explained to Punisher how he was able to track them to the World Trade Center.
After the OsCorp board of directors forced Norman Osborn out of the company Norman began to abduct the board members as Green Goblin. One of these board members also worked at the World Trade Center. As the Green Goblin, Norman used the Goblin Glider to fly several stories into the air to the board members office at the World Trade Center and abducted him. Spider-Man was later able to rescue all the board of directors from Green Goblin.
- In the episode Duel of the Hunters, Kraven the Hunter says that he was able to track Man-Spider to the garage of the World Trade Center by tracking the scent of gunpowder that was used in an explosion in the World Trade Center garage from several years ago. This is a reference to a real life terrorist attack that happened on February 26, 1993.
- After the September 11, 2001 terrorist attacks which brought down the twin towers all references to the World Trade Center were edited out of reruns.