The Hudson River is a 315-mile river that flows from north to south primarily through eastern New York in the United States.
When John Jameson's shuttle landed on the George Washington Bridge, Spider-Man went there to save him and Paul Stevenson. Spider-Man managed to save the two astronauts and the shuttle fell into the Hudson (with Spider-Man still inside). Spider-Man was able to make it to the surface and discovered a black slime on his costume. Spider-Man at first thought that it was pollution from the Hudson River. However, he later learned that the black slime was really an alien symbiote.
When Green Goblin kidnapped Mary Jane Watson he brought her to the George Washington Bridge. While Spider-Man and Green Goblin fought Mary Jane accidentally fell off the side of the bridge and through a time dilation portal. This trapped Mary Jane in limbo. However, Spider-Man believed Mary Jane had fallen into the Hudson River and dived in to save her. However, Spider-Man was unable to locate her.