When the Tri-Spider Slayer attacked OsCorp, Norman Osborn got into his car and drove to the George Washington Bridge to get away from it. Spider-Man later arrived at the George Washington Bridge and was able to jump on top of the Tri-Spider Slayer and place a bomb on it and when the bomb exploded it destroyed the Tri-Spider Slayer.
Sometime later astronauts John Jameson and Paul Stevenson lost control of their space shuttle and made a crash landing on the George Washington Bridge. Kingpin then sent Rhino to the bridge to retrieve the Promethium X from the shuttle. As Rhino arrived he fought Spider-Man but was able to get away with the Promethium X. Jameson and Stevenson were then taken to the hospital.
When Green Goblin learned Spider-Man was really Peter Parker, he kidnapped Mary Jane Watson and brought her to the George Washington Bridge to use as bait for Spider-Man. When Spider-Man arrived they fought and during their fight Mary Jane fell off the bridge and into a time dilation portal and ended up trapped in limbo.