S.H.I.E.L.D eventually discovered that the Chameleon had been hired by terrorist to sabotaging a peace treaty signing of two warring countries at the United Nations. Agent X was one of the undercover agents at the Daily Bugle and her mission was to protect the leaders and stop the Chameleon. When Nick Fury ordered that they get the leaders out of the Bugle, Agent X took them to the roof to get onto a helicopter to take them to safety. When Agent X, J. Jonah Jameson, and the VIPs got to the roof they were greeted by Nick Fury. However, Spider-Man was there on the roof and realized that Nick Fury's eyepatch was on the wrong eye and that he was really the Chameleon. When Spider-Man attacked the Chameleon the S.H.I.E.L.D. agents believed that he was attacking Nick Fury and tackled him and placed him in handcuffs. Spider-Man then yelled that Fury's eyepatch was on the wrong eye. When Agent X noticed that she held Chameleon at gunpoint, However, Chameleon was able to escape by hijacking a plane. As Agent X shot at the plane she hit it and the plane crashed into a river. Chameleon was later arrested.
- Agent X was an original character created for Spider-Man: The Animated Series. However, many fans believe that Agent X is really Sharon Carter or Black Widow who in the comics are also S.H.I.E.L.D agents.
- Agent X also shares some similarities with Maria Hill such as being portrayed as Nick Fury's right hand woman. However, Agent X can not be based off Maria Hill in any way because Maria Hill did not appear in the comics until 2005.